Physical DescriptionRecording spectrophotometer, Cary 14 dual beam ultraviolet (UV)-visible-near infrared, metal / plastic / glass / paper, chart recorder, lamp power supply, instruction manual and accessories, used by Biochemistry Department, University of Sydney, Sydney, New South Wales, Australia, 1960 to c. 1980, made by Applied Physics Corporation, United States of America, 1960
The unit is contained in a grey metal box, with a built-in chart recorder located over the spectrophotometer controls and lamp housing. The unit was operated while mounted on a standard desk type metal leg table with one drawer (not acquired), with the power unit for the lamp on a lower shelf of the support table. The lamps are water cooled and the lamp coolant flow switch assembly is included although disconnected from the unit. The internal valve based electronics of the chart recorder are reached by a rear hinged door. The university had a large and heavy refrigeration unit (weighing c100 kgs - not acquired) to control the sample temperature. The unit is otherwise complete and presumably in working order once the lamp cooling assembly is reconnected.
A detailed manual, titled "Cary Model 14 Recording Spectrophotometer Instruction manual", is with the unit. The title page of the manual lists the unit as a "Model 14-PM-50N, serial no. 332". Schematic drawings in the manual are printed with the date 9.10.1959 (being drawn in the USA this presumably means September 9, 1959).
Spare slide wire unit, with cables, for the chart recorder - in cardboard box
2 x 40mm quartz monochromator prisms - in small cardboard box
Partial roll of chart paper - in cardboard box
2 x plastic containers of blue ink
Spare chart recorder pen with attached ink reservoir
2 x double channelled [monochromator prism holder]
1 x single channelled [monochromator prism holder]
Box of assorted fuses and bolts
Specific types of molecules can be identified by the characteristic wavelengths of light they absorb or transmit. The spectrophotometer is used for measuring the absorbance or transmittance as a function of wavelength. The key components of any spectrophotometer include a light source, dispersion device, sampling area and one or more detectors. In recording (or scanning) spectrophotometers, a double-beam design permits measurement with continuous change in wavelength. A sample beam and a reference beam (aimed through a blank) are monitored continuously so that absorbance (or transmittance) can be calculated at each wavelength.
MarksTwo lines of black embossed self-stick plastic labelling tape is affixed above a knob located to the bottom left of the sample chamber. The first line reads, "IN FOR I.R." The second line reads, "OUT FOR VIS/U.V.".
One line of red embossed self-stick plastic labelling tape is affixed near the top of the door of the chart recorder and reads, " PLEASE FILL IN LOG BOOK".
The outside of the box containing the spare slide wire unit has the writing,
"0 - 0.1
0.1 - 0.2